Veterinary Recombinant Biologics

KindredBio is focused on next generation veterinary therapeutics: biologics, such as monoclonal antibodies and recombinant proteins.

Next Generation Therapeutics: Biologics

In humans, therapeutic biologics have revolutionized the practice of medicine, providing more effective treatments for many diseases including various cancers as well as inflammatory and autoimmune disorders.  KindredBio is focused on development of next generation veterinary therapeutic biologics for cats, dogs, and horses.

Monoclonal Antibodies

Monoclonal antibodies (mAb) are antibodies produced by identical cells cloned from a unique parent cell.  They bind to a specific epitope on the targeted cell type or soluble target.

Monoclonal antibodies have become the gold standard for treatment of many human diseases because of their safety and efficacy profile, along with their high specificity and long half-life. Anti-inflammatory monoclonal antibodies which inhibit TNFα, such as Infliximab (Remicade®) and Adalimumab (Humira®), are widely used in the treatment of Crohn’s Disease, Ulcerative Colitis, and Rheumatoid Arthritis to name a few. Rituximab (Rituxan®), a monoclonal antibody to CD20 on B cells, is used for the treatment of non-Hodgkin’s lymphomas. Other monoclonal antibodies including Bevacizumab (Avastin®) and Ranibizumab (Lucentis®) are widely used for treatment of inflammatory and autoimmune disorders as well as cancer in human medicine.

Recombinant Proteins

Recombinant biologics are produced by inserting the genetic code for a specific therapeutic protein into the DNA of an expression vector in the laboratory.  The DNA codes for protein which are inserted into the genome result in production of large quantities of recombinant protein through replication in living host cells in the laboratory.  The recombinant protein produced is then purified and the final preparation administered to the animal (cat, dog, or horse) for targeted therapy.  The final preparation (drug formulation) is sterile and contains a highly pure and targeted drug. One example of a recombinant protein commonly used in human medicine is erythropoietin alpha (Epogen®).

The Process

1 The process starts with identification and isolation of the genetic code for the desired therapeutic protein and inserting the genes of interest into the expression vector’s DNA.

2 The expression vector is double-stranded DNA capable of inserting itself into the host cell thereby introducing the genetic code of the therapeutic protein into the host cells.

3 The host cell produces recombinant protein along with host-cell proteins.

4 Recombinant protein is collected, separated, and purified from the host cell proteins and other components using conventional chromatography.

5 The final drug formulation is sterile and contains highly pure drug substance capable of binding to the specific epitope on the cell receptor or to a soluble target (such as cytokine).